Somatic Embryogenesis and Flow Cytometric Assessment of Nuclear Genetic Stability for Sansevieria spp.: An Approach for In Vitro Regeneration of Ornamental Plants

Sansevieria Thunb. species are traditionally known as succulent ornamental plants worldwide. They also cultivated for medicinal, fodder, soil conservation and fiber uses, their capacity to reduce environmental pollution. sexual propagation is limited by the lack of viable seeds, reproduction largely made via vegetative suckers or cuttings. For these reasons, genetic improvement conventional breeding limited. To overcome this problem address increasing demand from customers novel varieties, many commercial companies regularly use in vitro propagation, case process several plants. In paper, first time, we report a procedure somatic embryogenesis plant regeneration starting three flower explants seven different genotypes. Regeneration was attempted using stigmas/styles, anther/filament, ovary which were cultured on Murashige Skoog solidified medium under growth regulator combinations. A good rate obtained with all genotypes used culture conditions tested every explant type, percentages ranging 0 73.3%. “Genetic stability” assessment regenerated respect mother verified through flow cytometry analysis showing high degree uniformity, only S. parva exhibiting level DNA fluorescence among This an interesting achievement aim produce true-to-type new variants desirable characteristics, both desired features ornamentals improvement.